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Observation Possible Cause Action

Condensation may have been

present on the Digital PCR 20K

Chip during imaging.

IMPORTANT! Condensation

may not be visible on the Digital

PCR 20K Chip.

• If condensation is

not

visible

– If possible, read the Digital

PCR 20K Chip again after it

has warmed to room

temperature. Once

equilibrated, the data quality,

flag, and separation of

amplified versus non-

amplified peaks should

improve noticeably.

• If condensation is visible –

Using a low-lint wipe,

remove any condensation or

Immersion Fluid from the

surface of the chip by wiping

in one direction. If

necessary, use a low-lint

wipe sprayed with

isopropanol to remove any

dried residue.

The Sample Loading Blade was

not seated correctly within the

loader arm.

Before pressing the loading

button, confirm that the Sample

Loading Blade is firmly seated

and flush against the loader arm.

Chapter 5 Troubleshooting

Troubleshooting chip images using the Chip View

QuantStudio

3D Digital PCR System User Guide

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Quant 3D Q150 Guía de usuario Pagina 83